Catalase investigation essay

The higher the temperature, the higher the rate of reaction up to a certain point.

If the SD is a large number, it means the results are far away from each other, meaning your results are not that consistent. These are called fruitful collisions. This energy is the activation energy.

Be aware of pressure building up if reaction vessels become blocked. An intensive metabolic activity can be found in soaked pea seeds as they begin to germinate.

This cycle will continue until there is no Hydrogen Peroxide left. This is because enzymes are proteins and their structures get damaged when the temperature rises above 40 C. Conduct a pilot study to check acceptable concentrations of hydrogen peroxide for the investigation by measuring the quantity of oxygen evolved from a reaction in which a low concentration of hydrogen peroxide is treated with 3 parts lesser quantity of the enzyme Catalase.

The test tubes used must be of sealed type with a provision for inserting the probe of a suitable oxygen detector into it. If there is a small SD it means the results tend to be close to the average result, meaning that they are all relatively close together.

Then the rate of reaction is calculated. Preparation a Make just enough diluted hydrogen peroxide just before the lesson. Usually, there is only one active site on an enzyme; however there can be more. Also, if available lab coats will also be worn. Apparatus and Chemicals For each group of students: Hence, there is a chemical need to remove hydrogen peroxide in a very rapid rate.

Dilute immediately before use and put in a clean brown bottle, because dilution also dilutes the decomposition inhibitor. How could I measure the rate of reaction. This can be accomplished by using a heating block to carry out individual reactions for a standard amount of time.

Sets 3 and 4 were complicated by the fact that in both sets there were values that can be classified as anomalies, as they both deviated from the rest of the values in that set so much that they could not be considered when calculating the average, and must therefore be disregarded, as for some reason those trials failed.

Be sure to clamp the gas syringe securely but carefully.

The variable to be tested was the substrate concentration of the reaction, in this case the hydrogen peroxide. There will be used, a beaker of cm3 of water at the appropriate temperature into which will be immersed, the boiling tube containing the reactants once their temperatures have equalised and stabilised.

Role of Catalase in the Red Blood Cells, in prevention of toxicity from hydrogen peroxide. This can be explained by an equation: This graph shows that small pieces react better than bigger pieces.

Also, the same volume of water will be added to the beaker each time a new test is to be done, so as not have any differences in how long the water maintains its heat for.

Once the bonds have been broken, the enzyme is said to have become denatured. The steadily raising line in the beginning of the curve plotted represents the increased rate of catalysis by the enzyme Catalase. Is my conclusion valid?. Potato Catalase Lab Essay Grade 12 Biology (SBI 4U1) Nicole INVESTIGATION INTO THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITY AIM The aim of this experiment is to find out the effect of temperature on catalase or hydrogen peroxide.

This will enable us to tell at what temperature hydrogen peroxide is most efficient. The investigation has shown that the activity of catalase in potatoes can be affected by changes in different ph levels and hydrogen peroxide concentrations.

The reaction reached its optimum at 30% substrate concentration and ph 9 respectively. An Investigation Into The Effect Of PH On The Activity Of Potato Tissue Catalase Aim The aim of my investigation is to see how pH affects the activity of potato tissue catalase, during the decomposition of hydrogen peroxide to produce water and oxygen.

Catalase Investigation Essay - Catalase Investigation Introduction This investigation aims to determine the effect that varying the pH of the conditions in which a reaction between an enzyme and a substrate takes place.

Investigation - Hydrogen Peroxide An investigation into how the concentration of the substrate 'hydrogen peroxide' affects the rate at which it is broken down to oxygen and water by the enzyme catalase.

Investigation into the Enzyme Activity of Catalyze in the Decomposition of Hydrogen Peroxide Essay Sample. In this pre-designed procedure the enzyme activity of catalase in a potato was tested, in the form of measuring the volume of the Oxygen (O2) released by a catalase – hydrogen peroxide (H2O2) reaction in the span of one minute.

Catalase investigation essay