Identifying two unknown species of bacteria essay

It had a positive reaction meaning the bacteria produced the enzyme catalane. A stock broth culture of the two unknown species 72 was obtained. All those stains give you additional data on your unknown cell and brings you closer to knowing which species it belongs to.

In this article, I'm going to go over some of the simple tests you would perform on your unknown in order to identify it.

The acid fast stain differentiates bacterial cells with lipoidal cell call. The dying solution can be either basic or acidic. Pseudomonas fluorescens has one flagellum it is called montrichous and Proteus vulgaris has several flagella peritrichous.

If it doesn't grow, it is aerobe. You test this in a cystine trypticase agar tube with phenol red as an indicator.

As a result of the biochemical test performed on the two Unknown bacteria several species of bacteria could be eliminated as possible suspects.

Indeed, we have approximatively human cells and bacterials cells in us. The Gram stain produced satisfactory results with a clear indication that the yellow species was Gram positive and the red species was Gram negative.

Like in the thioglycolate broth, aerobes grow on top, facultative anaerobes grow everywhere but mostly on top and anaerobes grow everywhere.

Gram-positive cells, like Staphylococcus aureus, are purple under the microscope and Gram-negative cells, for example Escherichia coli or Neisseria subflava, turn out red. Cells are treated first with carbol fushin which is heat-fixed, then with acid alcohol which decoulorize all cells except acid fast bacteria and finally with a counterstain methylene blue.

Both contain agar, which is composed of complex polysaccharides, NaCl and yeast extract or peptone. The bacterium also colonizes the human mouth, mucosae, oropharynx and upper respiratory tract. Therefore, we encounter bacteria everywhere and it is sometimes necessary to identify them.

As a result of the biochemical test performed on the two Unknown bacteria several species of bacteria could be eliminated as possible suspects. Different bacterial species creates different culture morphologies.

The Voge-Proskauer tests shows the production of acetoin. The cells will appear blue and the cell wall red. It's mostly a technique to rule out some options and to make sure we are dealing with a bacteria and not for example a mold.

For example, species from the Staphylococcus phylum are round bacteria that grow in clusters. By assayer Choose Type of service. For unknown bacteria 1, the Gram negative results narrowed the possibilities down to E. Bacteria are everywhere, they are part of our environment and even of us.

The litmus milk test was performed on both Gram positive and Gram negative bacteria to differentiate bacteria based on various reactions that occur in skim milk supplemented with a litmus pH indicator.

The area around the burner can be considered sterile. The Litmus milk test resulted in Litmus reduction ruling out E. Two streaks were performed using loops of the stock broth on a fresh dish of nutrient agar and incubated for 48 hours 32C and 25C.

An example of a species with a cell wall is Flavobacterium capsulatum. The results show that glucose, lactose, and sucrose fermentation with acid accumulated in the test tube.

It was determined that M. There are two main types of bacteria, gram negative and gram-positive. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number The following are the tests that were used to identify the two different bacteria.

Identification of Two Unknown Bacteria Abstract The purpose of this experiment was to learn how to isolate and identify unknown bacteria using methods from the lab.

Unknown 9 was used for this experiment. /5(3). The Identification of Two Unknown Species of Bacteria Essay. There are many reasons for knowing the identity of microorganisms - The Identification of Two Unknown Species of Bacteria Essay introduction. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or.

Identification of Two Unknown Species of Bacteria by Anna Albrecht (Microbiology ) Introduction axonomy is defined as ―the science that studies organisms in order to arrange them into groups (taxa)‖ (Nester, ). Identifying Two Unknown Species of Bacteria Essay - Identifying Two Unknown Species of Bacteria Materials and Methods Week 1, Day 1 (10 November ) The first day an unknown sample was assigned to each group of students.

The first test applied was a gram stain to test for gram positive or gram-negative bacteria. Identifying Two Unknown Species of Bacteria Essay - Identifying Two Unknown Species of Bacteria Materials and Methods Week 1, Day 1 (10 November ) The first day an unknown sample was assigned to each group of students.

The first test applied was a gram stain to test for gram positive or gram-negative bacteria.

Identifying two unknown species of bacteria essay